Inhibition of Ribosomal Ribonucleic Acid Maturation in Rat Liver by 5-Fluoroorotic Acid Resulting in the Selective Labeling of Cytoplasmic Messenger Ribonucleic Acid
نویسندگان
چکیده
منابع مشابه
Inhibition of ribosomal ribonucleic acid maturation in Novikoff hepatoma cells by 5-fluorouracil and 5-fluorouridine.
5-Fluorouracil, at lop4 M concentrations, inhibited ribosomal RNA maturation only slightly in Novikoff hepatoma cells growing in suspension culture. On a molar basis, 5-fluorouridine was a much more potent inhibitor of the maturation process. The difference between the effectiveness of 5-fluorouracil and 5-fluorouridine is probably a result of the greater transport and incorporation of the latt...
متن کاملLocalization of deoxyribonucleic acid complementary to ribosomal ribonucleic acid and preribosomal ribonucleic acid in the nucleolus of rat liver.
The fraction and distribution of DNA complementary to ribosomal RNA and preribosomal RNA was investigated by the technique of molecular hybridization with the DNA of isolated nucleoli and nuclei of rat liver. Nuclear DNA complementary to ribosomal 18 S RNA and 28 S RNA was 0.014% and 0.032%, respectively. Nucleolar DNA complementary to ribosomal 18 S RNA and 28 S RNA was 0.10% and 0.22%, respec...
متن کاملStudies on Liver Messenger Ribonucleic Acid.
Strong evidence has accumulated in recent years in favor of the following scheme of protein biosynthesis. DNA directs the formation of complementary strands of messenger RNA which possess the necessary genetic information to determine the sequential arrangement of amino acids in polypeptide chains (1, 2). The messenger RNA attaches itself to ribosomes to form a multiple ribosomal structure, the...
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Human peripheral lymphocytes are stimulated to change, in vitro, from a resting state to one of progressive enlargement, culminating in mitosis, by treatment with Phaseolus vulgaris phytohemagglutinin. The sedimentation characteristics and fate of rapidly synthesized RNA have been studied in this system after 20 hours of stimulation with phytohemagglutinin, a time when RNA synthesis has been ac...
متن کاملThe genes for cytoplasmic ribosomal ribonucleic Acid in higher plants.
The genes for cytoplasmic ribosomal RNA are partially resolved from the bulk of the DNA by CsCl equilibrium centrifugation. Although in some plants the buoyant density of the ribosomal RNA genes is as expected from the base composition of ribosomal RNA, others show a large discrepancy which cannot be due to the presence of low G-C spacer-DNA. The cross-hybridization observed with 1.3 and 0.7 x ...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1971
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)34133-x